关键词: MHV, Elisa试剂盒, RT-qPCR, 比对实验

Abstract: Objective　Evaluate the performance of various mouse hepatitis virus (MHV) enzyme-linked immunosorbent assay (ELISA) kits, and compare the concordance rate of the detection result between ELISA and quantitative reverse transcription polymerase chain reaction (RT-qPCR) method.Methods In this experiment, 179 clinical serum samples of mice were used to compare the specificity, sensitivity, inter-batch and intra-batch differences, compliance rate and other performance differences between five Elisa kits and B kits. RT-qPCR method was used to detect 91 mice samples from tracheal+lung, liver and fecal samples, and the result were compared with the result of Elisa.Results　Using the test result of the B kit as A reference standard, the result showed that the A kit showed false positives in the negative samples and the C kit showed false negatives in the positive samples. The highest dilution gradient that the A and C Elisa kits could detect positive serum was both 2-5. The intra-batch and inter-batch differences of A kit were kept within 15%, and the intra-batch differences of C kit were kept within 15%, while the inter-batch differences significantly exceeded 15%. Compared with B kit, the positive coincidence rate of A kit was 96%, the negative coincidence rate was 98%, and the comprehensive coincidence rate was 97%. Compared with B kit, C kit had a positive coincidence rate of 93%, a negative coincidence rate of 92%, and a comprehensive coincidence rate of 92%. Secondly, the coincidence rate of MHV detection by Elisa and RT-qPCR was 69%, and the MHV positive result detected by RT-qPCR were all detected in fecal samples. Rest 3 kits did not show positive result. Conclusion　With the test result of B kit as the reference standard, Among the five MHV Elisa test kits, kit A had the best comprehensive performance, such as specificity, sensitivity, difference between and within batches and coincidence rate. The coincidence rate of MHV detection by Elisa and RT-qPCR was 69%. Rest 4 kits did not show positive result accurately.

Key words: MHV, Elisa kit, RT-qPCR, comparison test